The POCKIT™ system sounds like a game-changer for PCR amplification and detection. Using Insulated Isothermal PCR (iiPCR) technology, it simplifies the process by applying Rayleigh-Benard convection to drive PCR with a single heating source, reducing reaction time significantly compared to conventional PCR.
Key features include:
- Fast Reaction Time: iiPCR reduces cycles, speeding up the process
- Multiplex Detection: Up to two optical channels (520 nm / 550 nm) for detecting multiple targets
- User-Friendly: Touch panel monitor displays qualitative results without post-amplification manipulation
This technology seems ideal for rapid and qualitative nucleic acid detection.
Principles of iiPCR
Insulated Isothermal Polymerase Chain Reaction (iiPCR) technology provides isothermal heating at the bottom of special-designed capillary tubes to induce a natural thermal convection to drive the PCR reaction along with the temperature gradient in an insulated environment. This technology can reduce the reaction time significantly, and also inherits the advantages of sensitivity and specificity from PCR assay.
- The environment is insulated by radiation shell.
- Natural thermal convection is induced by heat.
- Thermal convection drives fluid cycling and results in PCR reaction.
- Denaturation of dsDNA.
- Primer annealing.
- Extension of new strand.
- DNA is duplicated with the completion of one cycle of thermal convection.
